Social policy Assignment Example | Topics and Well Written Essays - 1750 words

Social policy - Assignment Example The paper focuses on two policies that have the potential of solving problems created by welfare systems for single mothers with creation of sustainable employment opportunities and reduction of out of wedlock births being areas of concern. The number of children being raised by single mothers has continued to increase over the years with data indicating the ratio of children living in families headed by women at all-time high (Fang and Keane 2). Introduction of welfare programs for single mothers is informed by existing challenges they go through in being the only parent in the family. These mothers have to deal with increased demand for attention from their children, despite the fact that most of the mothers work in low paying jobs with few or no benefits (Deily 135). Apart from low income that is not sufficient to support the families, single mothers also have to work longer shifts to meet family needs. This implies that they have less time to take care of the children yet they cannot afford to employ a capable substitute housekeepers and caregivers (Albelda 200). Due to the continued rise in the number of single mother, there has been increased scrutiny of programs seeking to support single women with children. The program has been accused of not only improving the economic position of these women but also ensure they remain dependent on welfare. There is now a split in opinion over the best approach to tackle the situation especially when assessing the importance of welfare programs to economic stability of such families against the need to enable these women through polices that will make them economically independent in future. As a result of the arguments on the effectiveness of assistance to single mothers, there has been increased argument in support of polices that would empower women through job creation as opposed to focusing on welfare (Garfinkel and McLanahan). Due to the inherent challenges within

Should animals be used in Sports and entertainment Research Paper

Should animals be used in Sports and entertainment - Research Paper Example The side in favor of using animals for sports and entertainment present their views that involvement of animals in activities of sports and entertainment is a normal practice and it has been used for centuries in cultural activities. There are a number of cultural events in Spain and South America which use bulls who end up either running on the roads and striking whoever comes in their way, or trying to hit the waving cloth. These activities do not cause harm to the participating animals in any way and therefore there is nothing unethical about this. (Debatepedia) On the other side, the opposing side argues that such cultural activities are what promote the use of animals in a brutal way. They argue that humans tend to go a step further and involve the animals in a cruel game or way. According to them, if the use of animals in simple sports and entertainment is banned, then the brutal games will be finished itself. The side against the involvement of animals for means of sports and entertainment also argues that animals should also not be used for showing skills and displaying their trained nature to the public. Such activities are performed in the circus where the wild animals are trained to behave in an unnatural way of publicly accepted attitude by the force of a whip (RSPCA Victoria). Also they see the elephant rides as unethical as this means that the commercial purpose promoters are using the animals as a means to an end and not as an end themselves. This is according to the categorical imperative introduced by Immanuel Kant (Kant). In this context, it can be related to the fact that the animals are used for commercial purposes and not taken care of because they are animals. In response to this, the side in favor of utilization of animals for entertainment and sports present their views that the circus is where the public, particularly children, experience these animals. When the general public watches and experiences these animals, only then they fall in love with these animals. They argue that one can only love an object or living being if he or she watches and experiences it. When these people see these animals in circus or in zoo, they fall in love with them and then they take care of them. Therefore, usually circus and zoo turns out to be the first step of taking care of the animals on the part of general public and eventually falling in love with them. Most people do not go on safari rides or jungles to see the wildlife and therefore their only chance of watching these animals is in zoo or circus. Therefore, it is also fair to the humans to experience the gifts of nature in the shape of animals, and also there is nothing wrong in doing this. Humans who like to play and enjoy usually tend to feel good when they are involved in sporting activities. When a person who loves to play football play well end up in a good mood when it plays football. Also jolly people who like to enjoy feel happy when they are involved in an act of entertainment as this makes them and others laugh and enjoy. Similar to people are animals who also feel happy when they play and provide entertainment. Most of the animals have playful personalities and

Strategic Management.Changes In The Remote Environment Of U.S Assignment

Strategic Management.Changes In The Remote Environment Of U.S - Assignment Example Further, the essay illustrates a competitor profile for the American university versus the Stanford university and lastly, this essay elaborates on the outlines of the success of the Google firm and the industry analysis model to name but a few. 1.) Briefly describe two important recent changes in the remote environment of U.S. business in each of the following areas: a.) Economic b.) Social c.) Political d.) Technological e.) Ecological Having put that across, it is worth to note the pertinent changes that the U.S business has undergone so far. U.S’s remote environment on business has undergone through several changes if viewed under the following factors, economic, social, technological, ecological, and political. First is pertinent to understand that, the economic nature of a business involves the direction and nature of the economy in a given firm. Therefore, two factors that affect the remote economical changes of the business are first, the consumption patterns, where it is vital for each firm to consider the trends of its economy affecting the industry. Second, managers should consider general inflation rates, credit accessibility and the growth of the gross national product. Secondly, on social issues it can be noted that recently women have proved to be the majority entry in the labor markets. However, the above has not only affected the compensations, hiring, and employee’ resource capability but also it has created the expanded product demand. In addition, the second pertinent social alteration is the customer accelerating interest as well as quality life employees. Workers tend to demand salary increase, and other advanced opportunity, which is incorporated in the social status. On political side, it is pertinent to acknowledge the fact that stability as well as direction of politics are core factors that facilitate the evaluation of the remote environment. Example of the two factors is the government acts in supporting the competitorà ¢â‚¬â„¢s advantages and some actions of government. The above, can affect the firm’s remote environment, such actions are the customer, and supplier functions. On other hand, the technological issue also affects the remote environment of the business in U.S. Here, technological factor is essential in promoting the innovation and in avoiding the obsolescence; therefore it is vital for the firm to be aware of the industrial influence on the technology. Technological change may affect the new markets that are sophisticated and it can as well shorten the firm life in anticipation. Another factor is the ecological niche, here the experts discusses that the core factor in the environmental remote is relationships between ecology and business in a reciprocated manner. A major cause of concern here is the human activity in an industrial society, which is a threat to the support of life. It is disturbing to realize that global climate has been a huge change in the remote of business r ealm. Another pertinent area is the loss of biodiversity and habitat. According to the experts, it is pertinent to acknowledge the fact that change of fauna and flora is at high speed and this can lead to its extinction. 3.) Develop a competitor profile for American Public University System and Stanford University. Next, prepare a brief strategic plan to improve the competitive position of the weaker of the two colleges. A worth noting fact is that in any given firm, competitors play a great part in either ensuring the development of the business environment or brings a business to a halt. For instance, the American university and the Stanford University have their own competitors staged in a well-set profile. However, the

Difference and diversity in Ontario Schooling Thesis

Difference and diversity in Ontario Schooling - Thesis Example Despite this, there are discontent voices in the overall education system of Canada. Not enough is being done in the education system to be able to provide all rounded education to people of divergent cultures. It will be naive to think that by providing a ‘standard’ form of education to everyone would yield same desired results. The truth is culture and diversity plays a key role in the education of an individual. An individual is a product his surrounding and these surrounding dictates that adjustment should be made to accommodate, assimilate or just understand that particular individual. The Canadian system has been criticized for seemingly favoring the dominant culture at the expense of immigrant cultures. The dominant white culture seems to be the propagated and ‘ideal’ way of living being fronted by the educational quotas. The concept of ‘Educating across difference’ has had its fair form of challenges that have cultural connotations. In r eality it is not the cultural values themselves that bring out the difference in the education system, but it is the values that are attached to particular cultural practices that breed contention. To bury our heads in the sand and ignore issues centering on power and minority can greatly work to undue our delicate social fabric. Power related issues such as ethnocentrism, racism and sexism should form the basis of the discussion on the divergent cultural issue. To be able to understand and appreciate the urban educational system, we first have to learn a brief history of Canada. When faced with the question of diversity and difference, Canada has chosen five broad based responses, each determined by the ideology that was stronger at that specific time in the period. These five responses are; suppressing difference, insisting on the difference, denying difference, inviting difference and lastly critiquing difference. This model of response and framework have been granted by various scholars (Fleras and Elliot, 1992 and Murkerjee 1988; Sleeter and Grant 1944). 1. Suppressing Difference: Aggressive Assimilation The first framework of suppressing is an unsurprising form of human reaction when one meets diversity and divergent views. The dominant and the most widespread at the time take precedence and any new culture has to conform to the pre-existing one. The need for ‘civilize’ others saw some communities being forced to give up their language, religion and forced to embrace the superior French culture (Ashworth, 1993). This campaign of suppression was referred as aggressive assimilation and it was subjected on early immigrant families. The late 1800 brought more quests to ‘Canadize’ the huge numbers of immigrants arriving from Eastern and Southern European countries. This euphoria of the New Canada unwittingly gave rise of fascist and white supremacist movements, which had their support stemming, albeit innocently at first, from all li festyles. Social Credit and CFF parties had endorsed such views bringing together the newly formed the United Church of Canada (Jaenen, 1977, pg. 89). Theories of Social Darwism emphasized on the superiority of Christianity, Anglo-Saxon, Western industrialization and capitalism. Therefore in this sense education was to be used to eliminate the difference in culture and propagate the ‘right’ cultures. 2. Insisting on Difference: Separation and Segregation The

A Training Program for Persons with Mental Retardation Program Assignment

A Training Program for Persons with Mental Retardation Program - Assignment Example The test development can be traced back to the 1980s. In 1986, there was a trial version of it created by a student pursuing Masters Degree under one of the authors. The test operates on samples of the elderly population. In its first test, normative data was provided differently in groups of respondent ages, (50-69 and 70+ years) and the National Adult Reading Test Estimated IQ (NART-IQ) group (85-89,100-114,115 and above). This test was conducted on 186 men and women (50 to 96) inhabiting England, and the majority of those tested came from Bristol Area. Unfortunately, there is little validity evidence to underscore conclusions made from the LLT experimentation on the elderly people. As said before, the exam is purely conducted on the elderly people though not in groups but as solo individuals. The exam, however, faces criticism due to its minimal levels of validity in evidence purported to have been gathered and therefore not appropriate for clinical applications. (Coughlan. A Holl ows, S.p.22) 2 The Substance Abuse Subtle Screening Inventory (SASSI) is developed with the aim to identify people who are most probable to have substance dependence malady (is). The test can be of use in a forensic setting if one wants to know whether an individual’s behavior is dependent on substance dependence. It has been noted that behavioral traits of people dependent on substances vary a lot.     The test was authored by Miller, Glenn. A and published by The SASSI Institute. It has been reviewed in the book ‘’the fifteenth mental measurements yearbook’’ by Plake, Impara, and Spies (2003). The test includes face valid and items that are considered subtle that have no relationship with substance abuse. Materials used for the test includes guides that have instructions for administering, filling in the scores, interpreting and manuals providing information on validity, reliability and development. Examples of professionals’ inferences that may be extracted on the premise of scale scores include awareness of the negative effects of substance abuse, evidence of emotional pain, and risk of being in touch with the legal or judicial institutions and people. The test is applicable to adults and Adolescents between 12 and 18years.The SASSI’s room for easy administration, scoring and analysis and the availability of computer styles for relia ble storage of data and analysis makes the test instrument highly useful in producing valid results in research ventures. For example, the Adult SASSI-3 has helped identify persons who have high chances of substance dependence problem with a total objectively-tested accuracy of 93%.The Adolescent SASSI-A2 has a 94% overall tested accuracy. 3 The Wechsler Adult Intelligence Scale, 4th ed. (WAIS-IV) is used to examine IQ or Cognitive functioning and is designed to target adults who fall in the 16 to 90 years age bracket. The exam looks into cognitive ability using a basic group of 10 subtests that aim at four specific areas of intelligence: perceptual reasoning, verbal comprehension, working memory and speed of processing.

The Picture of Dorian Gray by Oscar Wilde Essay Example | Topics and Well Written Essays - 500 words

The Picture of Dorian Gray by Oscar Wilde - Essay Example Harry’s argument easily wins him over and he is totally changed person after the meeting. â€Å"The few words that Basils friend had said to him--words spoken by chance, no doubt, and with willful paradox in them--had touched some secret chord that had never been touched before, but that he felt was now vibrating and throbbing to curious pulses.† But the worldly and satanic wisdom of his mentor makes him break the heart of the innocent soul. He feels remorse at first but again justifies himself in the light of Machiavellian morality of Lord Henry. Lord Henry is the evil angel of the story .Every moment the hero is close to repentance, he appears from nowhere and through his devilish speech changes the mind of Dorian. One is wonderstruck at the callousness of both Henry and Dorian they show at the death of Sibyl. â€Å"What is done is done. What is past is past." The yellow book is another diversion provided to him by Lord Henry. This book further goads him to indulge in â€Å"all the passions and modes of thought that belonged to every century except his own.† Now Evil in him is in its true attire. Under his influence, the friends meet destruction. He murders his once beloved Basil as he exposed the ugliness of Dorian’s soul .The portrait is a surrogate of soul, so when he sins his soul is changed . Even in the end to avoid the pangs of consciousness, he resorts to opium. He seems to be following Henry’s philosophy â€Å"To cure the soul by means of the senses, and the senses by means of the soul.† When Dorian has ominous hallucinations about his possible end, all his fears are dispelled by Lord Henry who declares that destiny is too wise or cruel to send us omens. The bad influence of Lord Henry even subdues the feelings of guilt created in Dorian’s heart. His resolve to reform himself is diverted to some other thoughts. The damnation is complete .The inherent evil in the

Qualities of a nurse with a BSN as a Mentor Essay Example for Free

Qualities of a nurse with a BSN as a Mentor Essay Qualities of a nurse with a BSN as a Mentor The nurse I chose as a mentor has wonderful qualities that I hope to have after I also obtain a BSN. She shows great communication between nurses, doctors, and families. She is able to explain any situation in a professional manor. Her confidence is shown through her professionalism, for example, when she addresses information of medication and disease process to a family, she explains thoroughly, which builds trust with the family, shows her confidence and accountability, and she takes the time shows care. I know that being a nurse is a stressful job but nurses are able to find emotional stability and this nurse does just that by finding the balance of being happy in one room of a patient who was just extubated and doing well, then she will go to her next patient who isn’t doing as well and have empathy for the family, she says the right things, keeps her own emotions stable and nonjudgmental, and changes her whole thought process room to room. She pays attention to detail by noticing the wrong medication dose before giving to a patient. She has great problem-solving skills by helping residents and doctors come up with solutions in critical situations, she has quick responses with traumas. For example, she noticed something not right with her patient after a certain IV medication was given. She shows respect to all medical staff and families by staying professional, culture sensitive, and always shows a positive attitude. I have observed nurses with ADN and nurses with BSN’s, and I have noticed that BSN nurse show more professionalism in the hospital setting. She believes that nursing is a commitment of service and the desire to help those in need. It is more than treating an illness, she is focused on delivering quality patient care to each patient. Her philosophy of nursing combines the knowledge of medicine with compassion and respect. She has leadership skills that were learned through her education, patients are her number one priority, collaborates with the healthcare team, gives support, sensitive to patient’s needs, and shows praises to other colleagues. She works for the best interest for the patient, such as when she does not agree with an order written for a patient she will question the order and take action when needed. She also shows her leadership skills through forming  leadership meetings on ways to improve employee and patient satisfaction for our unit. She strives to improve areas on our unit that lack strength and finds solutions to make it better. Her attributes are what I am striving for my life long goals as I become a nurse with a BSN. The qualities that I have observed and the influence instilled through this nurse added with the education South University will be teaching me, will help me succeed as a professional nurse with a BSN. Not only will this add to my personal philosophy but also my own personal experiences as a current nurse and my willingness to continually learn, listen, accept advice, respect others beliefs, and work with others in any environment given (adaptability). I look forward to advancing my skill and succeeding as a professional nurse and continuing my education. Chitty, Black, K. (2007). Professional Nursing: Concepts Challenges (5th ed). W.B. Saunders Company.

Ebusiness Plan Online Furniture Shops Marketing Essay

Ebusiness Plan Online Furniture Shops Marketing Essay This report outlines an online business plan for our company known as the Online Furniture Shop. We tend to cater needs of several kinds of customers ranging from large organization, small offices, schools, restaurants, hospitals to homes. We offer a vast variety of products online on our website furniture-online.com. Consumers can not only browse through various categories of products, view their details and prices but they can also order furniture online. The rest of the report will determine several other elements required in the e-business plan for Online Furniture Shop. A.  Ã‚  Ã‚  The E-commerce Infrastructure Electronic commerce can be defined as the type of business or monetary transaction which involves the transfer of goods or services in exchange of some money over the internet. This covers a wide range of several types of businesses. These types include auctions, consumer based retail sites, music sites and business exchanges of goods/services between organizations. This business plan also determines the strategic plan required for establishing an Online Furniture Shop. The infrastructure of the business involves based e-commerce website development which will sell to both businesses and individually through the internet. The infrastructure of this e-business also comprises of DBMS, Web Master, Web-developer, E-marketing, logistics and distribution channels and integration of secure transaction channels.   B.  Ã‚  Ã‚  Ã‚  Ã‚  Markets in E-Commerce There are three types of markets in the e-business sector Business-to-Business, Business-to-Consumer and Consumer-to-Consumer. All the three business types have different business strategies and marketing plans since they focus on different groups of consumers. Our Online Furniture Shop will focus   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   a.  Ã‚  Ã‚  Business to Business Even though early e-commerce based businesses focused on retailing on the interest however it is believed that revenue generated through B2B e-commerce will exceed that of business to consumer revenue. A research published in 2000 stated that ratio of monetary volume of B2B to that of B2C is 10 to 1 (Rouse, 2010). Our company tends to cater the needs of large to small business organizations. We sell as wholesalers when dealing with businesses and selling in bulks. Our clients belong to categories such as schools, law firms, large business organizations, restaurants and other business class categories. We offer them furniture like office chairs, table, revolving chairs, folding tables, executive chairs, kindergarten chairs, hospital waiting room furniture etc. Organizations can view our products online and contact us through mail since we do not take order in bulk online rather we contact with the firm personally to fulfill their large requirements.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   b.  Ã‚  Ã‚  Ã‚  Ã‚  Business to Consumer Even though B2C e-commerce has gone through hard times especially after the dot.com bubble burst in 2000. However there has been an increase in the number of online shoppers since then. Even the future looks bright for B2C e-commerce as household shopping in U.S alone has raised from 39 to 48 percent in 2010 (Patton, 2007). Our other main clients are small scale consumers who want to furnish their homes. We provide high quality furniture like sofas, beds, dining tables, sofa-cum-beds, computer table etc. to our valuable consumers. The consumers can come on our website browse through the products and one they have selected what to buy they can register in simple steps, buy online through our highly secure website and get the delivery at their door-step within 5 business working days. Now buying furniture for home is just a few clicks away. C.  Ã‚  Ã‚  Ã‚  Ã‚  Business Models There are several e-commerce business models a company can integrate in their business plan for enhanced profitability. We will focus on merchant business model since we are the retailer and wholesalers of furniture further we also sale directly to our consumers through our website. Another model we might integrate is affiliation model in which the company might place banners on other websites to increase traffic on our website thus adapting pay-per-click traffic generation strategy. II. Marketing Concepts There are several innovative techniques through which our e-commerce business can market the offering and reach targeted markets. We will look individually analyze the strategies which will be used in our e-business.   A. Marketing Techniques Since the target market is individual consumer along with business sector therefore we will look at some of the ways in which Online Furniture Shop can position and market its offerings. Banners Company can place banners on popular websites with heavy traffic having an integrated link back to the site of the organization just like putting up billboards in real world. Crawlers We also intend to integrate SEO in our websites for search engines which use Crawlers technique to search the internet database so that the websites are ranked higher on the result page whenever a potential customer searches for furniture shops. Pop Up Pop-up ads can also be used as a marketing technique even though they are found quite annoying by some internet users. These are ads which will open up in a new window whenever a user opens an associated website like home decoration or office set-up. III. Communicating in e-commerce A. Social networking The use of social networking websites such as Facebook and Twitter for promotion of products and services will be quite beneficial for OFS since its a great way to bring customers to your websites and generate sales. These sites are also a great tool for link building by posting URLs which have a link to your website this method tends to increase the traffic (Introcaso , 2011). Through creation of social media our firm can offer discounts, coupons and announce contests to people following us on such social networking websites. Such sites are also a great tool to interact with the consumers directly and get feedback from them along with any suggestions. B.  Ã‚  Ã‚  Ã‚  Ã‚  Content Marketing OFS also intends to use content marketing as a method of disseminating and creating important content to acquire, engage and attract a potential target market. Content marketing basically is the art of communication with potential prospects and customers. The core essence of this marketing strategy is that if businesses keep giving consistent and valuable information to the buyer the buyer ultimately gives their loyalty as a reward. IV. Problems surrounding E-Commerce Due to a massive out growth in the e-commerce business sector there has also been an emergence of associated security threats. Due to which it is essential for e-commerce based businesses to cater some vital requirements. These fundamental security requirements of e-commerce business are the basis of a secure online business since this sector faces the greatest threat as stated by a report that the attacks in this sector have risen from 2006 to 2007 by 15% (Symantec, 2007). A. Privacy and security Since we also plan on starting an e-commerce based business therefore it is vital for the organization to integrate protection against potential security threats in the business plan.   We plan on carefully monitor and securing the monetary transaction channel before offering credit card purchase option like SSL and Address Verification Systems. Our website will also have a satisfactory site security, encryption, privacy policy and also insurance against identity theft if possible. V. Ethical Challenges in Online Business   Identity theft can be defined as the illegally acquired personal information like names, drivers license, address, Social Security Number, Bank or credit card number and exploiting this personal data for some unlawful activities (Sorbel, 2003). It is ranked as one of the fasted growing crimes in America.   Some laws and regulation which might regulate the rising issue of identity theft and which are also applied to our firm are described below. a. The Financial Services Modernization Act of 1999 According to the summary by The Federal Reserve Bank of Minneapolis (2000) this act has a clause which requires a disclosure of privacy policy by all financial institutions with regards to the sharing of personal information. This act has another requirement which states that a notice should be given to the consumer to opt out of sharing the personal information with some nonaffiliated third party. b. The Fair Credit Reporting Act This was proposed in order to secure the personal information of consumers which is exchanged during any business transaction. This act tends to promote the fairness, privacy and integrity of the information content of consumer reporting agencies as states by Federal Trade Commission (2011). VI. Conclusion Thanks to easy and cheap access to internet more and more people are buying online. Our e-business model caters almost all the requirements for establishing an online merchant business. The target market we are focusing on is also quite large therefore we expect a successful growth in the market.

Tetanus Toxin: Structure and Purification

Tetanus Toxin: Structure and Purification Tetanus is regarded amongst the most severe and fatal disease since ancient times [1]. Tetanus is termed from a Greek word ‘Tetanos’ which means- to contract *. The disease is generally initiated due to deep wounds, cuts, and during catastrophic situations like natural calamities, physical trauma, etc. [a] It was first discovered by Hippocrates in early 19th century *. However it was etiologically described by Carle and Rattone who were first to produce tetanus in animals by injecting pus extracted from an infected human with the same disease in 1884. During this same period Nicolaier also produced tetanus in animals from soil samples. Further research in 1889 by Kitasato revealed that animals were infected by this disease when they were injected with a particular organism isolated from a human patient *. Nocard in 1897 revealed that this disease can be treated by the use of its antitoxin. Moreover in 1924 the significance of toxoid came into existence during World War I which was formulated by Descombey and this passive immunization against tetanus was majorly used during World War II *. Structure of tetanus toxin: The tetanus toxin is of 150kD comprising of three fragments i.e. A,B and C having a molecular weight of 50kD each [n]. Fragments A and B were observed to be non-spastically toxic and also to block the release of catecholamine. It also inhibits the action of synaptic nerves and thus exhibits an important role in the toxicity of the toxin. On the other hand fragment C is regarded as the non-toxic subunit but retaining the required antigenic properties of the toxin. This fragment is seen to bind with gangliosides, motor endplates and synaptic membranes. It also helps in transportation of the toxin from the periphery to the central nervous system [o]. Krieglestein et al. in 1990 stated that tetanus toxin is a 151-kD protein. The complete amino acid sequence is known. The mature toxin is made of two peptide and contains 10 half-cystine residues. Treatment with 4-vinylpyridine in the presence of 6M guanidine converted six of them into s-pyridylethyl cysteine residues are determines by amino acid analysis. When alkylation was preceded by mercaptolysis, all 10 halfcystine residues were recovered in the s-pyridylethylated form. It was therefore concluded that the toxin contains six sulfhydryl groups and two disulfide bond [r] Mode of Action: Rossetto et al. in 2001 reported that the neuroparalytic syndromes of tetanus is caused by neurotoxins produced by bacteria of the genus Clostridium of 150 kDa proteins consisting of three-domains, endowed with different functions: neurospecific binding, membrane translocation and specific proteolysis of three key components of the neuroexocytosis apparatus. After binding to the presynaptic membrane of motoneurons, tetanus neurotoxin (TeNT) is internalized and transported retroaxonally to the spinal cord, where it blocks neurotransmitter release from spinal inhibitory interneurons. TeNT cleave specifically at single but different peptide bonds, VAMP/synaptobrevin, a membrane protein of small synaptic vesicles [s]. Kegel et al. in 2002 stated that the 50kD ligh chain subunit comprises of zinc metalloproteases which cleaves synatobrevin that is not involved in neuroexocytosis [t]. Foster in 2009 Stated that TeNT enters the body via wounds and initially binds and internalizes into the peripheral terminals of motorneurons where it is transported by retrograde axonal transport to the motorneuron in the spinal cord. TeNT is transported to somatodendritic postsynaptic sites and is released into the synaptic cleft where it undergoes receptor mediated uptake into the presynaptic termini of the inhibitory interneurons, from where it translocates into the cytosol and inhibits neurotransmitter release. [u]. Starting material for purification of tetanus toxin: Raynaud in 1951 developed a technique of using non-autolyzed toxin direct from the organism i.e. Clostridium tetani [i] .This technique gave an advantage of obtaining a more concentrated form of toxin as compared to that obtained from the filtrates [i][j]. For this purpose the organism was generally cultured and subcultured using Tarozzi medium* and modified Tarozzi medium [j] Latham medium was also widely used for the same reason [j] [k]. M. Matsuda et.al in 1989 also used modified Latham medium for culturing of Clostridium tetani [o]. Muller and Miller in 1954 investigated that pancreatic digest of casein contained some inhibitory content which was solved by charcoal treatment [y]. Toxin was also extracted by treating the bacterial cells in hypertonic solution using 0.1M sodium citrate and 1M sodium chloride as stated by Bernard Bizzini et.al [q] Conventional method to produce tetanus vaccine: The Harvard strain of Clostridium tetani is grown in a fermentor for about a week using a semisynthetic medium. This leads the bacteria to lyze and release the toxin obtained in the supernatant. This method yielded about 60-80 Lf/ml. This yield is then filtered and detoxified using formaldehyde. This reacts with the toxin molecule mainly the amino groups of lysineresulting in imine formation, further reacts with the unstable groups of imidazole or phenol ring finally involves a cross-linking reaction between the both the amino groups. Formaldehyde also affects the 3-D structure, therefore making the toxic conformational epitopes [y]. Purification by HPLC: Kunihiro Ozutsumiet.al. in 1985 used extracts from the organism for purification of tetanus toxin using High performance liquid chromatographic methods (HPLC) [j]. The toxin extracted from the previously described method was initially purified using ammonium sulfate precipitation followed by ultracentrifugation in order to get rid of the unwanted particulate matter by filtering it through a 0.2 um membrane filter. The concentrated sample in the equilibrating buffer at a pH of 7.5 proceeded through a final step of purification by running it on HPLC using a column of a TSK G3000 SW of 0.75 x 60 dimensions. This column was equilibrated using 0.1M sodium-phosphate buffer at a pH of 6.8 and the flow rate was maintained at 0.6 ml/min. The fractions obtained were tested for its protein content at 280nm using a UV spectrophotometer [j]. Further the efficiency of HPLC was compared with another gel filtration method using Ultrogel column [j] [o]. Purification using Sephadex G-100: For large scale production of tetanus toxoid, Alcohol precipitation was used for immunization purpose by Pillemer L. et.al [b]. However, Levine et.al in 1951 used to purify the tetanus toxoid by ammonium sulfate precipitation [c]. Later, further purification and characterization of the toxoid was achieved by filtering it through Sephadex gels using G-100 columns as stated by Williams C. et.al in 1965 [d]. This simplified and low cost method yielded four separable fractions of the toxoid where the first two fractions of 55-65% non dialyzable nitrogen possessed significant antigenic properties. The next fraction obtained was of smaller molecular weight and showed poor antigenecity when injected in animals; however the fourth fraction obtained was not identified but was predicted to be metabolic by-products of the organism and had no significant role [d]. Before running on the column the protein concentration was determined using a UV spectrometer at 280nm. Chromatographic gel filtratio n was performed using a column of 1.2 x 0.062 m dimension. The column was packed and equilibrated with 0.1M phosphate buffer with a pH of 8.5, additionally 1% formaldehyde can be added to inhibit the bacterial growth. The void volume after equilibration was maintained at 800ml at the flow rate was fixed at 80ml/hr. The sample loading volume was around 50ml and was concentrated to about 100,000 Lf. The four fractions were collected and were further seperated by recycling them on the same column [d]. This method gave an efficient insight on how to purify and separate different components of the toxoid. M Matsuda in 1989 carried out the separation of fragment A-B treated with urea by running it on a ccolumn packed with Sephadex G-25, equilibrated with 0.02M tris-HCL buffer containing trace amounts of dithiothreitol and urea [o]. Other gels such as Sepharose 4B and Sephadex G-200 was also used by researchers like Bernard Bizzini, Immunodiffusion test was also carried out using Ouchterlony’s method [o] [p] [q]. Papain Digestion of Tetanus toxin: Further research by Helting and Zwister in 1974 made possible to obtain fragment C from Tetanus toxin which has significant antigenic properties but lack pathogenecity and thus occupies a major role in immunization [e]. Helting et.al stated that Tetanus toxin can be degraded in a specific pattern. The mild papain digestion cleaves the F(ab) region. The papain enzyme breaks the 150kD toxin into two parts, one comprising of the C-terminal of the heavy chain i.e. of 47kD which corresponds to the Fragment C of the toxin whereas the other part of 95kD consists of N-terminal heavy chain subunit along with the lighter chain polypeptide forming the fragment B (refer to Figure 2). This Fragment B was observed to have a toxic effect on mice when injected with a sufficient dose and also has an adverse effect on the nervous system, thus it was necessary to purify and obtain only Fragment C for immunization and to further study its immune response [f]. The purified Fragment C was separated and ob tained by chromatographic methods and by using anti-Fragment C IgG [f]. Ulrich Weller in 1989 performed papain digestion for 16 hours of overnight stirring of the toxin at 25Â °C at a concentration of 40ug/ml. The toxin was suspended in 10mM sodium-phosphate buffer at pH of 6.5 with 1mM EDTA and NaN3 and 10mM cysteine. After the incubation period 0.5mM of Ll-chloro-3-tosylamido-7-amino-2-heptanone was added as a stop solution in order to inactivate papain by further incubating it at room temperature for 30 min and was then cooled to 0Â °C with saturated ammonium sulfate solution at pH 6.5 with further centrifugation. The precipitate was resuspended in the same buffer mentioned. This further proceeded for its separation and purification on Sephadex G-100 column and the fractions were collected at the flow rate of 15ml/hr b*. These fractions were further pooled and contrated using a Centiprep 10 concentrator and the buffer was changed to 0.5 M NaCl with 30mM Tris-HCl at pH 7.5. The fragments B and C showed up homogenously on SDS-PAGE. The fragment C was further dialyzed against 10mM sodium phosphate buffer at a pH of 7.5. The samples obtained were further checked for its protein content at 285nm and was determined by modified lowry method after trichloroacetic acid precipitation. They also ran an SDS-PAGE using rerducing and non-reducing gels and was stained by Coomassie blue-250 and the chains and fragments of the toxin were determined according to their known amino acid sequence b* Other methods developed to obtain fragment C: Fishman et al. (1992) Pointed out that the non-toxic binding fragment of tetanus toxin (fragment C) binds avidly to neural tissue and has a growing number of neurobiological uses. Its current utility is limited by both its high commercial cost and the complex procedure for its preparation requiring highly purified tetanus toxin. A short procedure was developed which prepares fragments of tetanus toxin from crude C. tetani extracts. The resultant proteins are atoxic with molecular sizes and immunological properties closely resembling fragment C. These proteins undergo retrograde axonal and apparent transneuronal transport in a fashion similar to fragment C [v]. Ledoux et al. in 1994 Indicated that tetanus toxin once internalized via receptor-mediated endocytosis, form membrane channels in order to traverse the endosomal membrane and enter the cytoplasm of the nerve terminal forming an association between neurotoxin monomers which results in an oligomeric form of the neurotoxin necessary for assembly of a channel through the hydrophobic interior of the endosomal membrane, thereby allowing passage of the neurotoxin or its active fragment through the resulting pore [w]. Technique used to test the specificity of the heavy and light chain subunits: Matsuda and Yoneda in 1975 isolated the heavy and light chain subunits from a toxin reduced by treatment with dithiothreitol-urea[g] [h]. Kunihiro Ozutsumiet.al. in 1985 used the technique of electrophoresis using sodium-dodecyl-sulphate polyacrylamide gel i.e. SDS-PAGE as shown in Figure (3). and was further used to put up a western blot in order to check the specificity of the isolated subunits obtained [l] [m] [j]. SDS-PAGE allowed the toxin to stack at 49kD corresponding to the fragment C subunit and 85kD comprising of the 4heavy chain subunit [j] Goretzki and Habermann in 1985 characterized enzymatic fragments of tetanus toxin by immunoblotting using a set of previously characterized antibodies and a set of novel antibodies. The selected antibodies recognized the light chain, fragment C (ÃŽ ²1) and the complementary piece (ÃŽ ²2) of the heavy chain when blotted on nitrocellulose. All toxin preparations contained intrinsic esteroprotease activity which became manifest in the presence of urea. The main product of papain hydrolysis is fragment C, which appears as a double band under non reducing conditions but is homogeneous when reduced. Chymotryptic digestion hydrolyses the heavy chain well but leaves the light chain largely intact. Tetanus toxin is very resistant against trypsin as compared with other proteases, although this enzyme splits numerous different links [x].

Smith Quotes :: essays papers

Smith Quotes Monopoly...is a great enemy to good management. The Wealth of Nations, Book I Chapter XI Part I p148 The monopolists, by keeping the market constantly understocked, by never fully supplying the effectual demand, sell their commodities much above the natural price. The Wealth of Nations, , Book I, Chapter VII, p63 The price of monopoly is upon every occasion the highest which can be got. The Wealth of Nations, , Book I, Chapter VII, p63 People of the same trade seldom meet together, even for merriment and diversion, but the conversation ends in a conspiracy against the public, or in some contrivance to raise prices. It is impossible indeed to prevent such meetings, by any law which either could be executed, or would be consistent with liberty and justice. But though the law cannot hinder people of the same trade from sometimes assembling together, it ought to do nothing to facilitate such assemblies; much less to render them necessary. A regulation which obliges all those of the same trade in a particular town to enter their names and places of abode in a public register, facilitates such assemblies... A regulation which enables those of the same trade to tax themselves in order to provide for their poor, their sick, their widows, and orphans, by giving them a common interest to manage, renders such assemblies necessary. An incorporation not only renders them necessary, but makes the act of the majority binding upon the whole. The Wealth of Nations, , Book I, Chapter X, p130 To widen the market and to narrow the competition is always the interest of the dealers... The proposal of any new law or regulation of commerce which comes from this order, ought always to be listened to with great precaution, and ought never to be adopted, till after having been long and carefully examined, not only with the most scrupulous, but with the most suspicious attention. It comes from an order of men, whose interest is never exactly the same with that of the public, who have generally an interest to deceive and even to opprress the public, and who accordingly have, upon many occasions, both deceived and oppressed it. The Wealth of Nations, p267 On competition: The natural price, or the price of free competition...is the lowest which can be taken, not upon every occasion indeed, but for any considerable time together.

Language Differences in America Essay -- Essays Papers

Language Differences in America America is a country of immigrants. Lots of people from other countries are coming here each year. Most of these people don’t know the language, so they face a lot of difficulties at first. Even after they’ve been in the country for quite a while they still have an accent and sometimes their grammar usage is not perfect. Often people, who were born and raised in the United States, cannot understand what people with different dialect or accent are saying, so that creates problems and uncomfortable situations for the immigrants. Sometimes people with an accent would be treated differently, as if they were not as intelligent or as smart as others. I am foreign myself and I can tell from my personal experience that it is really hard to start a new life in another country. Amy Tan in her â€Å"Mother Tongue† describes an example of the situation like this, when Amy Tan’s mother’s CAT scan results were lost and hospital would not apologize to her. All of this was because of Amy’s mother’s dialect, but when Amy, who speaks a perfect English, herself talked to the hospital â€Å" lo and behold – we had assurances the CAT scan would be found, promises that a conference call on Monday would be held, and apologies for any suffering my mother had gone through for a most regrettable mistake.† Sometimes it is necessary for the people to understand that person, who has a dialect or an accent, is not that much different from them and that person has to be treated equally, as well as someone without an accent. Different people come to America; they have different traditions, religion, lifestyles, and lots of other things, but all of them are trying to start a new life, because the one they had in the ... ...elf in the other persons place.† I think that she is right and we should have respect for each other and accent should not become a barrier for the communication. I agree with Thomas Bray that, â€Å"one becomes American by buying into a set of ideas honed by long — and often bitter — experience about what works and doesn’t work† but he also says that all of us need to assimilate to one culture and one standard, and personally I think it is not right and it can not happen, because if you are an immigrant, you cannot just ignore your whole past, but what would be better is to bring something special, bright and exciting from your past, and leave all the bad things behind. In this case both, the American society and you, would win. And our society has to help people do that, and it should not think of the people, who come here, as of unworthy their attention and respect.

Bad Decisions Essay

I have realized recently that friends should not have any affect at all on the decisions you make in life. It does not matter whether you are a middle school student, high school student, college student, or even a grown and mature adult. Making a wrong decision at the wrong time can have many negative affects on life, I know because I’ve been there. A wrong decision might only affect you temporarily or it could stay with you for the rest of your life. It could cost you a job or could keep you from getting into the college you dreamed of going to your whole life. The bad decisions are too much to risk. In a way, decisions should be made & held responsible only by the person making them. You should always think of how they will affect yourself and others. Never let a friend or anyone else force you to make a choice that could wind up hurting yourself or anybody. Always be careful, it could be something that could stick with you for life. Even think about how it could affect your family and friends. Would they be embarrassed by this choice? Would they be proud to tell other people about what you have done? Decision making can be seen as hard but also a very important skill. Some people may view it as an easy everyday thing, but to some it is harder. It is not always naturally apparent to some. My grandfather would say some people are born leaders and easily make decisions and some just are just not gifted in those areas. People should just make sure to evaluate the situation previous to making a decision that could hurt you or others. Taking advice from friends can be helpful, just think it through and make sure it is really the best thing for you too. But, keep in mind friends don’t always know what is best for you. Sometimes they don’t realize how it can affect you and they don’t even do this on purpose. I can even admit seeing myself doing it sometimes. Then again, this picture will fade when having fun, but make sure you always have it at reach in the back of your head. You never know when something could go wrong and ruin your life and opportunities. It is very important to always think of your future and what you want to do later in life. If you have always dreamed to go to medical school and become a OB/GYN don’t go out and under-age drink at a party and then go driving. You think now that you would never do anything like that, but in the moment you never know. Lots of people get caught in the moment and make a mistake thinking that most people get away with it. But if you go out there and drive drunk and kill a perfectly responsible person who has good judgment and makes good decisions you will be forced to live with that for the rest of your life. You will have to face their family face to face and tell them that you are the reason they lost their loved one. You also have to say how it was because of a bad decision. You just got caught up in the moment and wound up killing someone perfectly innocent. It goes from talking or being funny in class to something as serious as killing someone. Just imagine yourself in that scenario. When it comes to situations like this , nothing is funny anymore, it serious. It might seem that when you are young there is nothing to worry about, that you’re untouchable and nothing will happen to you, but really everything you do affects your future. Not only because of your actions at that point but by getting use to making careless decisions some people never grow out of the habit. Before you know it, at 21 they are still the same people they were when they were 15. Bad decisions affect everybody , not just the person responsible but everyone around.

Brave New World: A Linguistic Analysis

The novel Brave New World by Aldous Huxley tries to show us the extreme consequences of social planning. As Huxley makes it, it is a project begun in philosophy, and ending in a few philosophers exercising control over larger society in order to suppress philosophy among the generality. The remnant of philosophers has earned the wisdom that thinking is deleterious to human happiness and social stability. Therefore the overriding goal of the 27th century world community is to suppress the natural human inclinations.In effect the community is only of the small coterie of philosophers at the helm, for the rest of humanity is maintained at a bestial level of existence by their machinations. They are bred artificially, and then raised through constant conditioning, all designed to make them function on their animal instincts alone, and so that they abhor the least tendency to reflection. All are made sexually sterile, and then encouraged to dwell on the sexual act with promiscuous abandon .Care is taken that the promiscuous partners do not fall in love, for love arouses the noble tendencies, and theses are dangerous to the status quo. Otherwise, any substantial thought in nipped in the bud, for the inhabitants of this society are encouraged to take the soma drug at the least onset of a serious thought process. It has been worked out that a hierarchy is necessary for this society to function, and according a five-fold caste system has been applied to the make-up of this society – from the alphas and betas at the top, to the deltas and epsilons at the bottom.The breeding and conditioning takes place according to this scheme. It is a triumph of logic, and yet it is also the death of the human. This conflict is the central theme of the novel. The opening section of the novel presents to us this theme variously and in poignant fashion. This essay carries out a linguistic analysis of the opening chapter, which sheds light on the overall theme. The general impression given is that society has progressed very far, so that logic and science have completely prevailed.The Director of the Hatcheries is describing to some students the process of artificially breeding the citizens of this society. His account tells us that it is a highly advanced process, and the machinery seems to be functioning flawlessly. As he enters the fertilizing room, there are fifty Fertilizer staff immersed in their work, and so the group is met with a â€Å"scarcely breathing silence, the absentminded, soliloquising hum or whistle, of absorbed concentration† (Huxley 2004, p. 16). All the clues point to a highly sophisticated society working on the factory principle.The factory principle is so esteemed years are counted from the year that the industrialist Henry Ford brought out his first mass-production car, the model T, which was in the year 1914. The present year is said to be 632 A. F. – the latter stands for â€Å"after Ford† (Ibid 15). But the fact ory is producing human beings. The cold calculation that is involved in this process reminds us of death rather than life. The suggestion is that the genesis of human being is also a process whereby humanity dies. Therefore, the general atmosphere painted is deathly, cold and uninspiring.â€Å"A SQUAT grey building of only thirty-four stories† (Ibid, p. 15). describes the Hatcheries Central, and defines a drab setting, to juxtapose it against its momentous function. Winter conditions are maintained to preserve the eggs and the sperm, and winter is also intimated in a symbolic sense. â€Å"Wintriness responded to wintriness. The overalls of the workers were white, their hands gloved with a pale corpse-coloured rubber. The light was frozen, dead, a ghost† (Ibid). The general impression is that this is not a place of life, but of death.The conflict is also between knowledge and ignorance. We are privy to a society where the excess of knowledge has begotten its antithesis, which is a will to ignorance. The society is based on a highly philosophical design, and yet the philosophy behind it is not supposed to be known by the citizens, because the entire object is to eradicate thinking. Thus the motto of World Society is emblazoned on the top of the entrance to the Hatcheries: â€Å"COMMUNITY, IDENTITY, STABILITY† (Ibid). The only possible way for the entire world to live as one community is to suppress thought.Individuality is encouraged, but only in so far as it pertains to action in particular, and eschews all generalizations that stems of thinking. â€Å"Not philosophers but fret-sawyers and stamp collectors compose the backbone of society,† we are told by the narrator (Ibid). â€Å"Identity† here means that one is able to fulfill one’s own immediate inclination or instinct. Stability is the result of this non-thinking and instinct-driven existence. In short, the plan is for a bestial existence, and one acquires stability just as a species of animal is stable in its jungle abode.Yet the highest philosophy must coexist with this manufactured ignorance, because the ignorance must be manufactured by someone. The Director of the Hatcheries is among the tiny group of citizens that must know exactly what is going on, for they must process and maintain it. He is part of the highest caste, the Alphas, those who are privy to all knowledge. But the second highest caste, the Betas, must also have a working knowledge, because they take on the high supervisory roles of the running of this society. â€Å"Just to give you a general idea† (Ibid 16).the Director is wont to say as he provides instruction to the Beta students. They are not supposed to know, yet they must be able to do their work properly, and with a modicum of intelligence. The knowledge that they are provided is just enough to keep them happy. They apply the knowledge towards the particular work that they have to do. If they do their work prof iciently they have job satisfaction and financial reward, and ask for nothing beyond these. But the danger is that the knowledge is applied generally, and beyond the confines of the particular situation.Such application of knowledge disrupts the whole pattern, and defeats the object of society. â€Å"For particulars, as every one knows,† the narrator tells us, describing the logic of the Director, â€Å"make for virtue and happiness; generalities are intellectually necessary evils† (Ibid). The last observation is told from the point of view of the Director, and it is significant that he describes generalities as â€Å"intellectually necessary evils†. This is admitting that evil has not been eradicated from this society. It is present in the process, especially in the thought process that engenders the entire system.But the intellection that takes place is necessary, so it is not the philosophers at the helm who are evil. There is no indication in the novel that the World Controllers abuse the power that they have appropriated. They are portrayed as selfless, and as having no concern but the greatest good of society. They hold the secret knowledge that thought is evil, but the evil does not touch their own person, while they proceed with their intellectual designs on society. The evil is instead diffused throughout the system. The evil aspect of this society is the aggregate loss of humanity.Happiness has been bought, but the price paid for it has been essential humanity. The gift of humanity is the greatest gift, and thus the price paid is the ultimate one. For all its apparent contentedness, this society is intrinsically inhuman, and the descriptions of the process taking place in the Hatcheries Central point towards an inhuman existence. â€Å"I shall begin at the beginning† (Ibid). This is how the Director begins his instruction, trying to manufacture a solemnity in keeping with the enormity of what is taking place, which is huma n genesis on a massive scale.But his effort falls flat, and it seems nothing more than a facetious pun. We notice the same effort towards solemnity in all his words and gestures. But solemnity is not possible in the presence of such mundane processes, no matter that the object is human genesis. He tells them about the operation that removes the female ovaries, which are then kept functioning artificially in order to provides the human eggs. We are told that the donors act voluntarily, but we know that it is actually a hefty bait of â€Å"a bonus amounting to six months’ salary† that induces them (Ibid, p. 17).Both sperm and egg cells are maintained at the right temperatures, before arriving at the fertilizing room, where cylinders containing the eggs are manually dipped into the sperm to effect fertilization. We are shocked to witness human conception under such a shabby process as dipping cylinders is seminal fluid. The calculation is relentless. Not all the fertilize d eggs are not all treated the same. Those embryos that are destined to become Alpha citizens are accorded the best treatment. All other embryos are deliberately maltreated, to various degrees, so that they form the lower hierarchies, from beta to epsilon.The â€Å"Bokanovsky’s Process† is the euphemistic term to describe this crime. The deliberate damaging of embryos in indicative of the inherent inhumanity of this society. It is one human being maiming another who is at the most defenseless state of existence. The evil is thus inherent in the process itself. Not just on the philosopher at the top, the indictment somehow falls on society at a whole. References Huxley, Aldous. Brave New World and Brave New World Revisited. London: HarperCollins, 2004.